Among the large number of micrornas , the excessive expression of microrna-21 has been found in the serum of cancer patients, including medulloblastoma , glioblastoma , colon cancer , hepatocellular cancer , and breast cancer , when compared to the corresponding normal tissues. An increasing number of evidence has demonstrated that up-regulation of mir-21 was observed in breast cancer, esophageal cancer, gastric cancer, colorectal cancer, lung cancer, pancreatic cancer, ovarian carcinoma, and so on [20, 21, 22] in this review, mir-21 up-regulated in breast tumor tissue as compared with normal tissue is widely supported by a large number of studies conducted in different groups. Objective:the aim of this study was to investigate the serum level of microrna (mir)-21 in patients with osteosarcoma and its correlation with chemosensitivity and prognosismethods:mir-21 levels in sera from 65 patients with osteosarcoma and 30 healthy controls were measured by real-time reverse transcription-polymerase chain reaction. On the other hand, the two most promising qpcr candidate mirnas, circulating mir-21 and mir-155, which were consistently reported as 25 to 35-fold higher in breast cancer by independent groups, were directly contradicted by genome-wide results, showing that both were down in breast cancer. In recent years, multiple studies have shown that mir-21 was significantly upregulated in almost all types of cancers, including breast cancer, esophageal cancer, gastric cancer, colorectal cancer, hepatocellular cancer (hcc), pancreas cancer, glioblastoma, leukemia, b-cell lymphoma, cholangiocarcinoma, lung cancer, papillary thyroid cancer, head and neck squamous cell carcinoma (hnscc), tongue squamous cell cancer, cervical cancer and prostate cancer selcuklu sd, donoghue mt, spillane c.
Previous studies have illuminated that mir-21-5p was high levels and be able to promote tumor invasion, intravasation and metastasis in various types of cancers (including colorectal cancer, gc. In a separate case-case analyses restricted to the 21 mirnas, we found 7 mirnas with differential expression for women whose breast tumors differed by her-2 expression, and 10 mirnas with differential expression by nodal status mirna levels in serum show a number of small differences between women who later develop cancer versus those who. Results: the assay was sensitive for detection of mir-21 in 0625 μl of serum from breast cancer patients for differentiation of samples from patients with locoregional breast cancer from those from healthy donors, the odds ratio was 1796 and the area under the curve was 0721. Abstract: breast cancer is the second leading cause of cancer-related death in women the majority of breast tumors are estrogen receptor-positive (er+) and hormone-dependent the majority of breast tumors are estrogen receptor-positive (er+) and hormone-dependent.
Of mirna-21 expression in breast cancer represents a feature signifying aggressive disease related to high tumor grade and negative hormone receptor status [53, 54] over-expression of the mirna-21 is associated with breast cancers elevated levels of jagged-1 (jag1), a predicted mir-21 target, are implicated in estrogen receptor-nega-tive (er-) breast cancer [55. Direct serum assay for microrna-21 concentrations in early and advanced breast cancer abstract background - small noncoding rna molecules known as micrornas (mirs) are involved in the regulation of gene expression. Direct serum assay for microrna-21 concentrations in early and advanced breast cancer (2010) export of micrornas and microrna-protective protein by mammalian cells. The aim of this study is to determine whether microrna-21 (mir-21), a specific microrna implicated in multiple aspects of carcinogenesis, promoted breast cancer metastasis by regulating the tissue inhibitor of metalloproteinase 3 (timp-3) gene. Yan lx, huang xf, shao q, huang my, deng l, wu ql, zeng yx, shao jy (2008) microrna mir-21 overexpression in human breast cancer is associated with advanced clinical stage, lymph node metastasis and patient poor prognosis.
Women with metastatic breast cancer, especially csii, presented up-regulated levels of mir-183, mir-494 and mir-21, which were associated with a poor prognosis these mirnas therefore represent new risk biomarkers of breast cancer metastasis and may be useful for future targeted therapies. And microrna-21 in human gastric cancer kazuo motoyama 1,2 , hiroshi inoue 1 , koshi mimori 1 , fumiaki tanaka 1 , kazuyuki kojima 2 , hiroyuki uetake 2 , kenichi sugihara 2 and masaki mori 1,3. Our analysis of colon, kidney, and breast tumor rna, although limited for very few samples, suggests a more modest up-regulation of mir-21 expression in these tumors compared with glioblastoma multiformes ( fig 1b supplementary data 3. Background: one major impediment to improving the management of breast cancer is the current lack of tumor marker with sufficient sensitivity and specificity a growing body of evidence implicates the diagnostic potential of circulating mirnas in cancer detection mir-155 plays an important role in the pathogenesis of breast cancer.
Keywords: pregnancy-associated breast cancer, breast cancer, microrna, mir-21, pten introduction pregnancy-associated breast cancer (pabc) is defined as cancer of the mammary gland diagnosed during pregnancy, up to 1 year after delivery or at any time while the patient is lactating 1. Background: microrna-21 (mir-21) is up-regulated in tumor tissue of patients with malignant diseases, including hepatocellular carcinoma (hcc) elevated concentrations of mir-21 have also been found in sera or plasma from patients with malignancies, rendering it an interesting candidate as serum/plasma marker for malignancies. Genomics of microrna-21 mirn21 gene that is located along the long arm of chromosome 17 (17q231) in an intergenic region (figure1), starts at 57918627 and ends at 57918698 bp from pter residing within the tenth intron of the tmem49 (transmembrane protein-49) gene in the direction of transcription, [7. Mir-10b targets hoxd10, thereby promoting cell migration and invasion [8, 16, 17] mir-21 has been reported to be associated with invasive and metastatic breast cancer and regulates epithelial-to-mesenchymal transition (emt) and hif1a in breast cancer stem cell-like cells. Of the 32 paired samples analyzed, 25 breast cancer tissues displayed overexpression of mir-21 in comparison with matched normal breast epithelium additionally, incidence of lymph node metastasis closely correlated with mir-21 expression, suggesting a role for mir-21 in metastasis.
Original paper systematic analysis of microrna involved in resistance of the mcf-7 human breast cancer cell to doxorubicin guo-qing chen æ zhi-wei zhao æ hong-ying zhou æ. Detection of microrna-21 expression as a potential screening biomarker for colorectal cancer: a meta-analysis, asian pacific journal of cancer prevention, 2014, 15, 18, 7583 3 differential microrna expression by solexa sequencing in the sera of ovarian cancer patients, asian pacific journal of cancer prevention, 2014, 15, 4, 1739. Microrna-155, or mir-155, is a commonly over-expressed oncomir in human cancers in human breast cancer, it has been identified to target the gene which encodes for a protein called suppressor of cytokine signaling 1 (socs1.
Conclusion: these results show that mir-21 is an indicator of an aggressive breast cancer phenotype and that it may be a new therapeutic target in the treatment of breast cancer in the future. Microrna 21 also known as hsa-mir-21 or mirna21 is a mammalian microrna that is encoded by the mir21 gene mirn21 was one of the first mammalian micrornas identified the mature mir-21 sequence is strongly conserved throughout evolution.
Microrna in breast cancer tissues from primary tumors and from metastatic sites gabriel rinnerthaler 1,2,3,† , hubert hackl 4,† , simon peter gampenrieder 1 , frank hamacher 1 . The underlying mechanism of microrna-21 in gemcitabine resistant breast cancer cells: wu zhenhua, tao zhonghua, zhang jian, xie jie, hu xichun department of medical oncology, fudan university shanghai cancer center department of oncology, shanghai medical college, fudan university, shanghai 200032, china.